What are the 3 methods of genetic transfer in bacteria?

Humanity is in grave danger due to powerful antibiotics becoming ineffective in the treatment of multi-resistant bacteria, also called superbugs. In this simulation, you will learn about the powerful abilities of these superbug bacteria. Your task is to find out how they acquire the foreign DNA that allows them to become superbugs.

Beat Dr. One in the board game ”Superbugs!”

In this simulation, you will try to beat Dr. One in a board game. This board game isn’t like other board games, however. Here, you will learn about the spread of bacterial infection and try to prevent a global pandemic! Dr. One is the enemy, trying to wipe out the human race, and it’s your task to save them! While playing this game, you will learn the key concepts of horizontal gene transfer in bacteria.

Learn the basics of bacterial genetics

First, you will learn about mutation and genetic elements of bacteria, which include chromosomes and plasmids. You will recreate the famous Microbial Evolution and Growth Arena (MEGA) plate developed by Harvard University and Technion-Israel Institute of Technology to highlight the inheritance of antibiotic resistance across many generations of bacteria. You have the freedom to decide where to grow your culture and how to set the antibiotic concentration levels. In the process, you will strengthen your understanding of the concept of mutation and its relation to the development of antibiotic resistance in bacteria.

Perform bacteria culture experiments

The spread of antibiotic resistance is not only a result of inherited random genetic mutations. By analyzing a patient’s sample with a resistant strain of Acinetobacter baumanii, you will be able to identify three methods of horizontal gene transfer that the bacteria can use to acquire foreign DNA. You will set up agar plates with your sample and controls, and complete a series of small learning games to understand the mechanisms and concepts behind conjugation, transformation and transduction.

Dive into the microbe realm

After completing the experiments and learning activities, you will take a virtual leap into the microscopic world. With bacteria, plasmids, and bacteriophages floating around you, your task is to find out which type of horizontal gene transfer will occur, based on your observation on the bacteria’s characteristics and surrounding environment.

Will you be able to grasp all the learning concepts behind the acquisition of antibiotic resistance? And will you be able to prevent Dr. One from winning the board game and wiping out the human race? We hope so!

Prokaryotes, which include bacteria and single-celled microorganisms called Archaea, usually pass their chromosomal DNA on to their offspring asexually. In other words, a bacterial cell reproduces by simply replicating its chromosome and dividing into two daughter cells. The daughter cells that result from this division are genetically identical to each other and to the original parent cell. Thus, over time, asexual reproduction in bacteria can lead to a population of hundreds of thousands of cells, all of which are genetically identical to a lone original parent cell.

Given their asexual method of reproduction, it is tempting to think that bacteria are sorely lacking in genetic variation, but this is not the case. Prokaryotic cells have developed a number of methods for recombining their genetic material, which, in turn, contributes to their genetic diversity. The three most common ways that bacteria diversify their DNA are transformation, conjugation, and transduction. However, not all types of bacterial cells are capable of engaging in all three processes.

Transformation

Transformation is a process by which a susceptible or "competent" bacterial cell acquires new genetic material from its environment. There are two types of transformation: natural and artificial. But where does the environmental DNA required for transformation come from? And how does this DNA become part of a bacterium's genome?

Natural transformation, as its name implies, is a natural mechanism used by some bacterial cells to take up DNA from the environment. This environmental DNA was, at one point, located in other bacteria. For instance, when bacteria die and disintegrate, their chromosomal DNA is released. Fragments of this DNA remain in the environment and are freely available to other living cells, including other bacteria. These naturally occurring DNA fragments can enter a living bacterium through its cell membrane, after contact with that membrane. If the DNA is double stranded, one of the strands will pass across the cell membrane into the cell, and the other strand will be dissolved, or hydrolyzed. Parts of the newly introduced single-stranded DNA molecule may then recombine with similar regions on the bacterial chromosome and become incorporated into the bacterium's genome.

In contrast, during artificial transformation, DNA uptake by bacterial host cells occurs under certain laboratory conditions. In the lab, scientists often introduce foreign DNA into bacterial cells via transformation in order to study specific genes and their functions. Typically, these researchers use E. coli cells that have been chemically treated so that their outer cell membranes are permeable to foreign DNA. In addition, transformation can be induced by electroporation, a process in which the bacterial host cells are subjected to an electric field that allows molecules to pass more easily across the membrane. Heat shock is another way that transformation can occur, wherein host cells are exposed to extreme temperatures that also cause the cell membrane to temporarily allow molecules of foreign DNA into the cell.

Within the lab environment, bacteria are also commonly transformed with sequences of DNA called plasmid vectors. These naturally occurring DNA molecules are circular, and they can replicate inside a bacterium independent of the bacterial chromosome (which can also be circular). Plasmid vectors can be used to clone, transfer, and manipulate genes. Often, these plasmids carry a gene for antibiotic resistance, which means that researchers can select for cells that are resistant to a given antibiotic in order to determine whether a bacterium has been successfully transformed.

The following animation depicts the process of transformation:

Conjugation

Conjugation is a process by which one bacterium transfers genetic material to another bacterium through direct contact. During conjugation, one of the bacterial cells serves as the donor of the genetic material, and the other serves as the recipient. The donor bacterium carries a DNA sequence called the fertility factor, or F-factor, on a circular, double-stranded plasmid. The F-factor allows the donor to produce a thin, tubelike protuberance called a pilus. The donor uses the pilus to contact the recipient. The pilus then shortens and draws the two bacteria together, at which time the donor bacterium transfers genetic material to the recipient bacterium. This genetic material is in the form of a plasmid, or a small, circular piece of non-chromosomal DNA. The newly transferred genetic material often provides the recipient bacterium with some sort of genetic advantage. In many cases, conjugation results in the transfer of a plasmid containing an antibiotic resistance gene.

Transduction

Finally, transduction is a process by which a virus transfers genetic material from one bacterium to another bacterium. This process depends on a specific type of virus called a bacteriophage, which is capable of infecting bacterial cells and using them as hosts to produce more viruses.

As the first step, a bacteriophage injects its DNA into a host bacterium. The bacteriophage DNA then takes over the host cell's machinery and directs it to synthesize and assemble more bacteriophages. During this process, the host cell's DNA breaks into fragments; after that, the host cell replicates the bacteriophage DNA and assembles new bacteriophages. Occasionally, some of the bacterial host cell's DNA is packaged in new bacteriophages during assembly. Once bacteriophage assembly is complete, the bacterial cell breaks open, and the newly assembled bacteriophages are released into the environment. Transduction occurs when a bacteriophage containing bacterial DNA infects a recipient bacterium and transfers this bacterial DNA to the recipient bacterial host cell. This transferred bacterial DNA may then be incorporated into the genome of the recipient bacterium.

What are the 3 mechanisms of gene transfer in prokaryotes?

These elements are transferred between organisms through different mechanisms, which in prokaryotes include transformation, conjugation, and transduction.

What are the methods of gene transfer between bacteria and describe each?

Genetic exchanges among bacteria occur by several mechanisms. In transformation, the recipient bacterium takes up extracellular donor DNA. In transduction, donor DNA packaged in a bacteriophage infects the recipient bacterium. In conjugation, the donor bacterium transfers DNA to the recipient by mating.

What are the three methods of genetic transfer in bacteria quizlet?

The terms conjugation, transduction, and transformation are used to describe three different natural forms of genetic transfer between bacterial cells.

What are the ways in which bacteria can transfer from?

5 Common Ways Germs are Spread.
Nose, mouth, or eyes to hands to others: Germs can spread to the hands by sneezing, coughing, or rubbing the eyes and then can be transferred to other family members or friends. ... .
Hands to food: ... .
Food to hands to food: ... .
Infected child to hands to other children: ... .
Animals to people:.

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